Effects of enhanced muscle growth by myostatin propeptide trangene and dietary fat content on gene expression of adiponectin, adiponectin receptors, PPAR-α and PPAR-γ

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dc.contributor.authorSuzuki, Shana T.N.
dc.date.accessioned2011-07-22T00:07:54Z
dc.date.available2011-07-22T00:07:54Z
dc.date.issued2007
dc.description.abstractMyostatin, a member of the transforming growth factor-β superfamily, is a highly conserved negative regulator of muscle growth which undergoes post-translational modification to yield the active form. We have demonstrated that transgenic over-expression of myostatin propeptide dramatically enhanced skeletal muscle development and decreased fat mass. By feeding the transgenic mice either a high-fat diet or normal fat diet we found that transgenic, high-fat diet mice had improved insulin sensitivity, normal fat deposition, enhanced muscle growth, and significantly higher levels of circulating adiponectin compared to wild-type mice. Adiponectin is known to ameliorate insulin resistance and increase fatty acid oxidation. We theorized that the interaction between high-fat diet and myostatin propeptide would increase adiponectin mRNA expression in fat tissue depots and corresponding adiponectin receptor mRNA expression in muscle and liver. Results from real-time PCR analysis indicated transgenic mice fed a high-fat diet displayed increased adiponectin mRNA expression in epididymal fat by 2- fold over wild-type littermates. These mice also displayed increased expression of PPAR-α and PPAR-β above the other three groups in epididymal fat. The wild-type mice fed a normal-fat diet expressed the most AdipoR1 and R2 in liver tissue, 1.03-fold and 1.32- fold over the other groups, respectively. The transgenic mice fed a high-fat diet did not show increased mRNA level of either receptor in muscle or liver tissue. The increase in expression of adiponectin mRNA may partially explain why the high-fat diet did not cause obesity and insulin resistance in transgenic mice.
dc.description.degreeM.S.
dc.identifier.urihttp://hdl.handle.net/10125/20774
dc.languageeng
dc.publisherUniversity of Hawaii at Manoa
dc.relationTheses for the degree of Master of Science (University of Hawaii at Manoa). Nutritional Sciences; no. 4186
dc.rightsAll UHM dissertations and theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission from the copyright owner.
dc.subjectTransgenic mice--Feeding and feeds
dc.subjectTransgenic mice--Endocrinology
dc.titleEffects of enhanced muscle growth by myostatin propeptide trangene and dietary fat content on gene expression of adiponectin, adiponectin receptors, PPAR-α and PPAR-γ
dc.typeThesis
dc.type.dcmiText

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