DNA Vaccine Expressing Mature Dengue Virus-Like Particles and Serological Tests to Distinguish Dengue and Zika.

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dc.contributor.author Youn, Han Ha
dc.contributor.department Biomedical Sciences (Tropical Medicine)
dc.date.accessioned 2019-05-28T19:35:07Z
dc.date.available 2019-05-28T19:35:07Z
dc.date.issued 2017-08
dc.identifier.uri http://hdl.handle.net/10125/62207
dc.title DNA Vaccine Expressing Mature Dengue Virus-Like Particles and Serological Tests to Distinguish Dengue and Zika.
dc.type Thesis
dcterms.abstract Our study investigated DNA vaccines expressing mature dengue virus (DENV) viruslike particles (VLPs) and developed serological tests to distinguish dengue and Zika. We showed that human-codon optimized DNA vaccines improved the expression and production of VLPs in vitro compared with non-codon optimized DNA vaccines. Human-codon optimized DNA vaccine expressing mature VLPs induced similar level of anti-DENV and neutralizing antibodies compared with that expressing mixed VLPs. Secondly, we found that DNA-prime and inactivated virion-boost regimens improved the immunogenicity in mice compared with DNA vaccine alone. Future challenge experiments are needed to show the protective effect of these vaccine candidates. Thirdly, we demonstrated that combination of three enzyme-linked immunosorbent assays based on non-structural protein 1 can distinguish DENV and Zika virus infections with high specificity and sensitivity. In light of the cross-reactivity of current serological tests, our findings have implications for serodiagnosis between DENV and Zika virus or other flaviviral infections.
dcterms.description M.S. Thesis. University of Hawaiʻi at Mānoa 2017.
dcterms.language eng
dcterms.publisher University of Hawaiʻi at Mānoa
dcterms.rights All UHM dissertations and theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission from the copyright owner.
dcterms.type Text
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