Genetic organization of a cluster of genes involved in the production of phaseolotoxin, a phytotoxin produced by Pseudomonas syringae pv. phaseolicola and sequence analysis of the phtE locus
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University of Hawaii at Manoa
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Phaseolotoxin, Nδ(N'-sulfo-diaminophosphinyl)ornithylalanylhomoarginine, produced by Pseudomonas syringae pv. phaseolicola, the bean halo blight pathogen, is a potent inhibitor of ornithine carbamoyltransferase (OCT). Inhibition of OCT in infected plants leads to chlorosis and growth inhibition. A genomic cosmid clone, pHK120, containing a 25 kb fragment of DNA from a wild-type strain of P. syringae pv. phaseolicola restores toxin production to Tox- mutants. Tn5 mutagenesis of pHK120 and marker exchange of pHK120
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Theses for the degree of Doctor of Philosophy (University of Hawaii at Manoa). Botanical Sciences (Plant Pathology - Cell, Molecular, and Neurosciences); no. 3036
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