EPIGENETIC TARGETS AND DOWNSTREAM EFFECTS OF NONI AQUEOUS EXTRACT ON ANTI-CANCER ACTIVITY

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dc.contributor.advisorMaunakea, Alika
dc.contributor.authorHead, Tony
dc.contributor.departmentCell and Molecular Biology
dc.date.accessioned2023-02-23T23:57:19Z
dc.date.available2023-02-23T23:57:19Z
dc.date.issued2022
dc.description.degreeM.S.
dc.identifier.urihttps://hdl.handle.net/10125/104673
dc.subjectMolecular biology
dc.subjectCellular biology
dc.subjectDNA Methylation
dc.subjectHCT116
dc.subjectNoni
dc.titleEPIGENETIC TARGETS AND DOWNSTREAM EFFECTS OF NONI AQUEOUS EXTRACT ON ANTI-CANCER ACTIVITY
dc.typeThesis
dcterms.abstractThis study aims to analyze methylation levels of genomic loci in HCT116 colon cancer cells altered by exposure to aqueous Noni extract for 48 hours. A Polynesian ethnobotanical with anti-cancer properties, Noni is a complex assembly of chemical constituents that influence DNA methylation states in cancer cells; we hypothesize that by analyzing and correlating DNA methylation and RNA expression pattern changes induced by Noni treatment, we will identify signaling cascades or pathways that explain decreases in cell viability, membrane integrity, and adhesion. Our focus is on the combined effects of Noni fruit in aqueous solution that contribute to the upregulation of gene expression and the downstream pathways potentially leading to the observed morphological and cell inhibitory effects on HCT116 cells. We identified multiple differentially expressed genes involved in proliferation/survival pathways, plasma membrane integrity, and cell adhesion. However, the direct influences of DNA methylation and expression were more complex; we conclude that differential DNA methylation and other factors likely interact indirectly to induce observed effects on expression, and consequently to anti-cancer activity. The identification of specific molecular pathways modulated by Noni has wide implications with respect to commercial production and harvesting, in addition to altering treatment options for a wide array of illnesses and diseases including cancer. Noni fruit is relatively abundant in Hawaii and can be prepared using simple methods. In addition, continued studies may further elucidate effects of variations in commercialized Noni products.
dcterms.extent75 pages
dcterms.languageen
dcterms.publisherUniversity of Hawai'i at Manoa
dcterms.rightsAll UHM dissertations and theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission from the copyright owner.
dcterms.typeText
local.identifier.alturihttp://dissertations.umi.com/hawii:11617

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