EPIGENETIC TARGETS AND DOWNSTREAM EFFECTS OF NONI AQUEOUS EXTRACT ON ANTI-CANCER ACTIVITY
Date
2022
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Abstract
This study aims to analyze methylation levels of genomic loci in HCT116 colon cancer cells altered by exposure to aqueous Noni extract for 48 hours. A Polynesian ethnobotanical with
anti-cancer properties, Noni is a complex assembly of chemical constituents that influence DNA
methylation states in cancer cells; we hypothesize that by analyzing and correlating DNA
methylation and RNA expression pattern changes induced by Noni treatment, we will identify
signaling cascades or pathways that explain decreases in cell viability, membrane integrity, and
adhesion. Our focus is on the combined effects of Noni fruit in aqueous solution that contribute to
the upregulation of gene expression and the downstream pathways potentially leading to the
observed morphological and cell inhibitory effects on HCT116 cells. We identified multiple
differentially expressed genes involved in proliferation/survival pathways, plasma membrane
integrity, and cell adhesion. However, the direct influences of DNA methylation and expression
were more complex; we conclude that differential DNA methylation and other factors likely
interact indirectly to induce observed effects on expression, and consequently to anti-cancer
activity.
The identification of specific molecular pathways modulated by Noni has wide
implications with respect to commercial production and harvesting, in addition to altering
treatment options for a wide array of illnesses and diseases including cancer. Noni fruit is relatively
abundant in Hawaii and can be prepared using simple methods. In addition, continued studies may
further elucidate effects of variations in commercialized Noni products.
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Molecular biology, Cellular biology, DNA Methylation, HCT116, Noni
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75 pages
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