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ANALYSIS AND DEVELOPMENT OF MANAGEMENT TOOLS FOR ORYCTES RHINOCEROS (COLEOPTERA: SCARABAEIDAE)

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Title:ANALYSIS AND DEVELOPMENT OF MANAGEMENT TOOLS FOR ORYCTES RHINOCEROS (COLEOPTERA: SCARABAEIDAE)
Authors:Adams, Brandi-Leigh H.
Contributors:Melzer, Michael (advisor)
Tropical Plant Pathology (department)
Keywords:Entomology
Agriculture
Plant sciences
coconut rhinoceros beetle
haplotype
show 4 morehigh-throughput sequencing
pheromone
qPCR
virus discovery
show less
Date Issued:2019
Publisher:University of Hawai'i at Manoa
Abstract:Oryctes rhinoceros (Coleoptera: Scarabaeidae) is a pestiferous beetle causing severe damage to coconut and other palms by boring into and feeding upon the crown tissue. Several tools are used to manage this beetle, including pheromone lured traps and the biocontrol agent, Oryctes rhinoceros nudivirus (OrNV). The pheromone lure, ethyl 4-methyloctanoate, was assessed for its attractive ability towards the CRB-S and CRB-G haplotypes by comparing the distribution of the haplotypes in non trap-caught and trap-caught populations collected from Palau. A diagnostic qPCR assay was designed to detect OrNV, and was found to be a more sensitive and accurate method for OrNV detection compared to the conventional PCR assay. Finally, because the newly emerged CRB-G haplotype has acquired resistance to OrNV, RNA extractions were sent for high-throughput sequencing to discover other viruses present in O. rhinoceros. Three sequences were discovered to be similar in sequence to viruses of other insect species.
Description:M.S. Thesis. Ph.D. Thesis. University of Hawaiʻi at Mānoa 2019
Pages/Duration:102 pages
URI:http://hdl.handle.net/10125/63272
Rights:All UHM dissertations and theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission from the copyright owner.
Appears in Collections: M.S. - Tropical Plant and Soil Sciences


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