ANALYSIS AND DEVELOPMENT OF MANAGEMENT TOOLS FOR ORYCTES RHINOCEROS (COLEOPTERA: SCARABAEIDAE)

dc.contributor.advisor Melzer, Michael
dc.contributor.author Adams, Brandi-Leigh H.
dc.contributor.department Tropical Plant Pathology
dc.date.accessioned 2019-07-02T18:04:30Z
dc.date.available 2019-07-02T18:04:30Z
dc.date.issued 2019
dc.description.degree M.S.
dc.identifier.uri http://hdl.handle.net/10125/63272
dc.subject Entomology
dc.subject Agriculture
dc.subject Plant sciences
dc.subject coconut rhinoceros beetle
dc.subject haplotype
dc.subject high-throughput sequencing
dc.subject pheromone
dc.subject qPCR
dc.subject virus discovery
dc.title ANALYSIS AND DEVELOPMENT OF MANAGEMENT TOOLS FOR ORYCTES RHINOCEROS (COLEOPTERA: SCARABAEIDAE)
dc.type Thesis
dcterms.abstract Oryctes rhinoceros (Coleoptera: Scarabaeidae) is a pestiferous beetle causing severe damage to coconut and other palms by boring into and feeding upon the crown tissue. Several tools are used to manage this beetle, including pheromone lured traps and the biocontrol agent, Oryctes rhinoceros nudivirus (OrNV). The pheromone lure, ethyl 4-methyloctanoate, was assessed for its attractive ability towards the CRB-S and CRB-G haplotypes by comparing the distribution of the haplotypes in non trap-caught and trap-caught populations collected from Palau. A diagnostic qPCR assay was designed to detect OrNV, and was found to be a more sensitive and accurate method for OrNV detection compared to the conventional PCR assay. Finally, because the newly emerged CRB-G haplotype has acquired resistance to OrNV, RNA extractions were sent for high-throughput sequencing to discover other viruses present in O. rhinoceros. Three sequences were discovered to be similar in sequence to viruses of other insect species.
dcterms.description M.S. Thesis. University of Hawaiʻi at Mānoa 2019
dcterms.extent 102 pages
dcterms.language eng
dcterms.publisher University of Hawai'i at Manoa
dcterms.rights All UHM dissertations and theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission from the copyright owner.
dcterms.type Text
local.identifier.alturi http://dissertations.umi.com/hawii:10232
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Adams_hawii_0085O_10232.pdf
Size:
2.35 MB
Format:
Adobe Portable Document Format
Description: