Temporal and vertical variability of ammonia oxidizing archaea in the subtropical north Pacific Ocean

Simple item page
dc.contributor.authorWai, Brenner Robert Kee
dc.date.accessioned2016-05-02T23:22:08Z
dc.date.available2016-05-02T23:22:08Z
dc.date.issued2013-12
dc.description.abstractWe sought to investigate environmental controls on two phylogenetically distinct Marine Group I (MGI) ammonia-oxidizing Thaumarchaea at Station ALOHA, the field site for the Hawaii Ocean Time-series (HOT) program. Samples were collected throughout the water column (0-4000 m) on near-monthly cruises between 2006 and 2012. We utilized quantitative PCR amplification of ammonia monooxygenase subunit A (amoA) genes and gene transcripts to evaluate temporal variability in the abundances, transcriptional activities, and vertical distributions of ammonia-oxidizing MGI at Station ALOHA. We observed three to four orders of magnitude differences in the depth distributions of these organisms, with peak abundances occurring between 125 and 300 m. Our results revealed amoA gene abundances underwent moderate increases in the epipelagic waters (0-100 m) during periods of deeper winter mixing. MGI amoA genes near the base of the euphotic zone (100-200 m) were elevated in the fall and winter, suggesting seasonal decreases in irradiance in this region of the water column could partly regulate abundances. Examination of MGI amoA mRNA revealed peak transcriptional activity near the base of the euphotic zone (125-175 m). Our results provide new information on processes influencing the distributions and physiological activities of microorganisms mediating nitrification.
dc.description.degreeM.S.
dc.identifier.urihttp://hdl.handle.net/10125/100763
dc.languageeng
dc.publisherUniversity of Hawaii at Manoa
dc.relationTheses for the degree of Master of Science (University of Hawaii at Manoa). Oceanography.
dc.subjectenvironmental controls
dc.subjectThaumarchaea
dc.subjectmicroorganisms
dc.titleTemporal and vertical variability of ammonia oxidizing archaea in the subtropical north Pacific Ocean
dc.typeThesis
dc.type.dcmiText

Files

Original bundle
Now showing 1 - 2 of 2
No Thumbnail Available
Name:
Wai_Brenner_r.pdf
Size:
3.05 MB
Format:
Adobe Portable Document Format
Description:
Version for non-UH users. Copying/Printing is not permitted
Download
No Thumbnail Available
Name:
Wai_Brenner_uh.pdf
Size:
3.11 MB
Format:
Adobe Portable Document Format
Description:
Version for UH users
Download

Collections

M.S. - Oceanography