Determination of Nuclear Dna Content in Orchids by Flow Cytometry
Files
Date
1996
Authors
Contributor
Advisor
Department
Instructor
Depositor
Speaker
Researcher
Consultant
Interviewer
Narrator
Transcriber
Annotator
Journal Title
Journal ISSN
Volume Title
Publisher
Volume
Number/Issue
Starting Page
Ending Page
Alternative Title
Abstract
Flow cytometric analysis was found useful for determining ploidy and DNA content values for orchid species and hybrids. Immature leaf tissue yielded the most reliable results for all orchids sampled. Leaf endopolyploidy was common, with levels as high as 16N found in mature D. gouldii leaves. DNA content values for 37 Dendrobium species, 11 Dendrobium hybrids, 33 additional orchid species and 8 hybrids were determined. Values for Dendrobium species ranged from 1.53 pg to 4.23 pg/2C nuclei. C-values for the remaining orchid species ranged from 1.91 pg to 15.19 pg/2C nuclei, with those of Cattleya alone ranging from 3.29 pg to 9.29 pg/2C nuclei. The highest variation within Cattleya corresponded to different specimens of the same species, suggesting polyploidy. An analysis of Dendrobium hybrids and parent species showed that DNA content values could be reliably predicted in cases where parentage was indefinite.
Description
Keywords
Citation
Extent
Format
Geographic Location
Time Period
Related To
Related To (URI)
Table of Contents
Rights
Rights Holder
Local Contexts
Collections
Email libraryada-l@lists.hawaii.edu if you need this content in ADA-compliant format.