Utility of RAPD Markers in Evaluating the Status of the Hawaiian Tree Fern Cibotium x heleniae
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2001-04
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University of Hawai'i Press
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Abstract
Randomly amplified polymorphic DNA (RAPD) markers provide
data consistent with the conclusion based on morphological characters that the
recently named taxon Cibotium xheleniae is indeed of hybrid origin. This assertion
is supported by (I) placement of C. xheleniae intermediate to the parent
taxa, as determined by genetic similarity data; (2) location of C. xheleniae individuals
on a clade intermediate to the parent species in the cladistic analysis;
and (3) clustering of the C. xheleniae individuals between clusters of parental
individuals in principal components analysis. Additivity of parental genetic
markers in the putative hybrid ranged from 54 to 64%, providing additional
though modest support for the hypothesized origin of C. x heleniae. Our results
indicate that RAPD data can be of considerable value in assessing potential hybridity
of individuals in naturally occurring populations.
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Motley TJ, Morden CW. 2001. Utility of RAPD markers in evaluating the status of the Hawaiian tree fern Cibotium x heleniae. Pac Sci 55(2): 145-155.
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