Decontamination of Melicope explants

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dc.contributor.authorKikuchi, Michelei K.
dc.date.accessioned2011-07-22T00:15:17Z
dc.date.available2011-07-22T00:15:17Z
dc.date.issued2008
dc.description.abstractMelicope is a genus mostly endemic to the Hawaiian Islands. Two of its species, M. anisata and M. paniculata produce berries that are coveted for leis. Although not endangered, Melicope populations have been impacted by wild collection of the fruits. The plants are propagated solely by seeds which take a long time to germinate. Melicope has not been successfully propagated by air layering and cuttings; micropropagation was used to try to obtain plants in vitro. The plants are only found in the wild, which made the plant material extremely hard to decontaminate. In an attempt to obtain sterile explants efficiently, different surface sterilization treatments included ethanol, hydrogen peroxide, sodium hydroxide, Plant Preservative Mixture™ (PPM), soapy water, chlorine gas, and antibiotics such as carbenicillin, amphotericin, streptomycin, and mefenoxam. Chlorine gas was the most effective at producing a high number of clean explants, while the use of PPM and antibiotics also gave good results. The explant type that had the highest decontamination rate were the microcuttings, which were also the tissues that were the most responsive in vitro. These finding may lead to a successful protocol for further research in the micropropagation of Melicope.
dc.description.degreeM.S.
dc.identifier.urihttp://hdl.handle.net/10125/20915
dc.languageeng
dc.publisherUniversity of Hawaii at Manoa
dc.relationTheses for the degree of Master of Science (University of Hawaii at Manoa). Tropical Plant and Soil Sciences; no. 4317
dc.rightsAll UHM dissertations and theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission from the copyright owner.
dc.titleDecontamination of Melicope explants
dc.typeThesis
dc.type.dcmiText

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