Prevention of microbial and biofouling attachments in food processing applications using nanoengineered surfaces

Simple item page
dc.contributor.authorRungraeng, Natthakan
dc.date.accessioned2015-10-02T20:58:18Z
dc.date.available2015-10-02T20:58:18Z
dc.date.issued2014-12
dc.description.abstractL. monocytogenes (1.9×108 CFU/mL) was used for bacterial adhesion testing of nanoengineered stainless steel surface, which may be more demanding for hygienic food processing equipment. The NS stainless steel surface was achieved by electropolishing the steel in a mixture of sulfuric and phosphoric acid. For NR stainless steel fabrication, the bare substrates were anodized in a mixture of perchloric acid and ethylene glycol to create two surfaces with two different in nanopore diameters ( 50 nm and 80 nm), so-called NP50 and NP80, respectively. Thereafter, the NS, NR50, and NR80 stainless steels were exposed to the L. monocytogenes solution in a stagnant environment for four hours. The enumerated average cell counts were 5×103, 5.0×101, and 2.8×101 CFU/cm2 for NS, NR50, and NR80, respectively. The results suggested that regardless of the substratum materials, nanoporous surface finishes could significantly inhibit the adhesion and attachment of L. monocytogenes. However, the statistical analysis indicated that the numbers of cells adhering to the 50 nm and the 80 nm nanoporous surfaces were not significantly different. The finding could be because besides the pore diameter, other surface parameters such as peak heights (or pillared magnitudes) and interpore distances might also play an important role on anti-adhesion of L. monocytogenes.
dc.description.degreePh.D.
dc.identifier.urihttp://hdl.handle.net/10125/101133
dc.languageeng
dc.publisherUniversity of Hawaii at Manoa
dc.relationTheses for the degree of Doctor of Philosophy (University of Hawaii at Manoa). Molecular Biosciences and Bioengineering.
dc.rightsAll UHM dissertations and theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission from the copyright owner.
dc.subjectnanoengineered surfaces
dc.titlePrevention of microbial and biofouling attachments in food processing applications using nanoengineered surfaces
dc.typeThesis
dc.type.dcmiText

Files

Original bundle
Now showing 1 - 2 of 2
No Thumbnail Available
Name:
Rungraeng_Natthakan_r.pdf
Size:
2.46 MB
Format:
Adobe Portable Document Format
Description:
Version for non-UH users. Copying/Printing is not permitted
Download
No Thumbnail Available
Name:
Rungraeng_Natthakan_uh.pdf
Size:
2.52 MB
Format:
Adobe Portable Document Format
Description:
Version for UH users
Download

Collections

Ph.D. - Molecular Biosciences and Bioengineering