Microbial diversity and community structure determinations through analyses of SSU rRNA gene distributions and phylogeny

Date

1995

Contributor

Advisor

Department

Instructor

Depositor

Speaker

Researcher

Consultant

Interviewer

Narrator

Transcriber

Annotator

Journal Title

Journal ISSN

Volume Title

Publisher

University of Hawaii at Manoa

Volume

Number/Issue

Starting Page

Ending Page

Alternative Title

Abstract

The majority of this study focused on the analysis of diversity and community structure through an examination of small-subunit (SSU) rRNA genes from the microbial mat assemblage located at an active, hydrothermal vent system, Loihi Seamount, Hawaii. The habitat studied was Pele's Vents, a deep-sea hydrothermal ecosystem located near the summit of Loihi Through a restriction fragment length polymorphism (RFLP) distribution analysis of a SSU rDNA clone library generated by the polymerase chain reaction (PCR), it was determined that two operational taxonomic units (OTUs) dominated the bacterial assemblage of the mat community. These two OTUs together accounted for ~73% of the bacterial SSU rDNA clone library examined, with ten OTUs accounting for the remaining ~27%. A technique, analogous to rarefaction, was developed to determine that diversity had been sufficiently described by the clones examined. SSU rDNA fingerprinting of clones belonging to each OTU was conducted to confirm OTU specificity and SSU rDNA identity. The phylogenetic diversity of the bacterial OTUs discovered at Pele's Vents (abbreviated PVB, for Pele's Vents Bacteria) was also described. Two dominant phylotypes were found that included more than a single OTU, and therefore comprised a cluster of phylogenetically related taxa The most abundant phylotype was the PVB OTU 2 cluster, which was comprised of PVB OTUs 2, 3, 6, and 8. The PVB OTU 2 cluster accounted for ~61% of the bacterial clone library and had a lineage contained in the ε-Proteobacteria subclass and a Thiovulum-like phylogeny. The second dominant phylotype was the PVB OTU 1 cluster, which consisted of PVB OTUs 1 and 11 and accounted for ~27% of the bacterial clone library. The PVB OTU 1 cluster had a lineage within the γ-Proteobacteria subclass and a Xanthomonas-like phylogeny. The remaining five PVB OTUs, each determined by a single clone, were represented by a wide variety of phylotypes spanning the domain Bacteria. The phylogenetic diversity of the archaeal OTUs discovered at Pele's Vents (abbreviated PVA, for Pele's Vents Archaea) were also described. The archaeal clones were dominated by the PVA OTU 2 cluster (comprised of PYA OTUs 2, 3, and 4), which was phylogenetically contained in the Crenarchaeota, whereas PVA OTU 1 was contained in the Euryarcheota. Both of these lineages were phylogenetically affiliated with recently discovered cosmopolitan marine archaeoplankton.

Description

Keywords

Microbial mats, Microbial diversity, Marine sediments--Microbiology

Citation

Extent

Format

Geographic Location

Hawaii--Loihi Seamount

Time Period

Related To

Theses for the degree of Doctor of Philosophy (University of Hawaii at Manoa). Oceanography; no. 3271

Related To (URI)

Table of Contents

Rights

All UHM dissertations and theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission from the copyright owner.

Rights Holder

Local Contexts

Email libraryada-l@lists.hawaii.edu if you need this content in ADA-compliant format.