Evidence for sap1 as a Virulence Factor in Burkholderia cepacia Complex

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dc.contributor.author Bluhm, Andrew
dc.contributor.department Microbiology
dc.date.accessioned 2019-05-28T20:14:54Z
dc.date.available 2019-05-28T20:14:54Z
dc.date.issued 2017-08
dc.identifier.uri http://hdl.handle.net/10125/62544
dc.title Evidence for sap1 as a Virulence Factor in Burkholderia cepacia Complex
dc.type Thesis
dcterms.abstract Burkholderia cepacia complex (Bcc) is a consortium of at least 20 closely related Gram negative species that are a risk factor for cystic fibrosis (CF) patients. Previously in B. pseudomaelli, a hypothetical protein with no known function, was identified to be a novel virulence factor and involved in attachment. In this work, highly conserved homologs in Bcc K56-2 and LO6 were examined in multiple in vitro and in vivo models such as attachment to eukaryotic cell lines, biofilm attachment and formation, Caenorhabditis elegans survival model, Drosophila melanogaster feeding model, and mouse lung infection. We found that the deletion mutants had impaired attachment and biofilm formation, and significantly lower in vivo survival and replication, compared to the wildtype strains. Finally, C. elegans and mice infected with the mutants had better survival compared to wildtype infections, supporting the hypothesis that the protein surface attachment protein 1, or sap1, is a virulence factor.
dcterms.description M.S. Thesis. University of Hawaiʻi at Mānoa 2017.
dcterms.language eng
dcterms.publisher University of Hawaiʻi at Mānoa
dcterms.rights All UHM dissertations and theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission from the copyright owner.
dcterms.type Text
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