Identification of a Rubredoxin-like Protein Required for Biotin Synthesis in Mycobacteria
Date
2022
Authors
Contributor
Advisor
Department
Instructor
Depositor
Speaker
Researcher
Consultant
Interviewer
Narrator
Transcriber
Annotator
Journal Title
Journal ISSN
Volume Title
Publisher
Volume
Number/Issue
Starting Page
Ending Page
Alternative Title
Abstract
In Mycobacterium tuberculosis (TB), biotin is crucial for synthesizing glucose, fatty acids, and mycolic acid components of the bacterial cell wall essential for survival and pathogenesis. TB grows in a biotin-deficient environment during infection and must synthesize its own biotin de novo. Biotin synthase catalyzes the final step in biotin synthesis, in which S-adenosylmethionine (SAM) is used to oxidize C-H bonds and a sulfur atom from an iron-sulfur cluster is inserted between two carbon atoms in the biotin precursor, dethiobiotin. In E. coli, biotin synthase also requires the exogenous electron donor flavodoxin, but this protein will not support the activity of the TB enzyme. In mycobacterial genomes, the biotin synthase gene (BioB or Rv1589 in TB) is found adjacent to two uncharacterized genes that code for small proteins with unknown functions (Rv1590 and Rv1591 in TB). Rv1590 codes for a metal-binding protein homologous to rubredoxin domains, suggesting Rv1590 could be involved in electron transfer reactions. This research project focuses on cloning the Rv1590 gene, heterologous expression in E. coli, purification of the resulting metalloprotein, and investigation of the secondary structure, thermal stability, metal content, and redox activity of the purified protein.
Description
Keywords
Chemistry
Citation
Extent
74 pages
Format
Geographic Location
Time Period
Related To
Related To (URI)
Table of Contents
Rights
All UHM dissertations and theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission from the copyright owner.
Rights Holder
Local Contexts
Collections
Email libraryada-l@lists.hawaii.edu if you need this content in ADA-compliant format.