Gut microbial communities of Plutella xylostella vary among Hawaiian island populations
Date
2024
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Abstract
It is well-established that for many species of Lepidopterans, population, along with various associated environmental effects such as host diet and local environments, has a significant influence over the host's community composition. Nonetheless, many insecticide-mediated resistance studies focus solely on lab-reared resistant lines, looking for taxa that convey resistance through functionality or an increase in abundance, often neglecting multi-population studies. Thus, the impact of population on the host's microbial response to insecticide application is poorly understood. This project seeks to identify if population impacts the bacterial diversity and community composition of the highly insecticide-resistant insect Plutella xylostella by analyzing the bacterial communities of field-caught specimens from several populations across Oʻahu, Hawaii and then rearing an F1 generation in the lab to check for dissimilarities in the bacterial communities among control populations and among populations exposed to sublethal and lethal doses of Spinosad. Our results showed that population strongly influences bacterial community composition and richness, with abundant taxa varying among populations and each lab-reared population exhibiting a distinct community composition. Moreover, when subsets of the lab populations were exposed to lethal and sublethal doses of Spinosad, the distribution of bacterial taxa varied among populations, with no uniform pattern. These results emphasize the importance of accounting for population-specific factors in insecticide resistance research and the necessity of integrating field and laboratory studies to better understand the role of the microbiome in insecticide resistance.
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Entomology, Insecticide resistance, Lepidoptera, Microbial ecology, Plutella xylostella, Population, Spinosad
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34 pages
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