MICROBIAL COMMUNITY HERITABILITY IN LARVAE OF THE HAWAIIAN CORAL MONTIPORA CAPITATA

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2024

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Coral health depends on microbial interactions that support functions such as organic material cycling and disease resistance. Microbial communities in planulae and adult tissues of Montipora capitata were examined in factorial fertilization crosses to investigate the heritability of microbial community characteristics and specific microbial taxa in this common Hawaiian coral. I hypothesized that larval microbiomes would be predicted more by the dam (egg donor) than the sire (sperm donor), consistent with reports of egg-transmitted algal symbionts in M. capitata. A factorial cross of gametes from five parent colonies produced 18 viable sibling families after self-crosses and low viability crosses were removed. Amplicon sequencing of the 16S rRNA gene profiled the composition and diversity of Bacteria and Archaea among adults, seawater, and planulae, defining a consistent microbiome community characteristic of planulae. Taxa found in the seawater were rare to undetected in the larvae suggesting horizontal transmission was low. Significant differences were found among larvae when grouped by either parent, indicating both dam and sire influenced the microbiome of the planulae; siblings were also more similar than half-siblings or unrelated larvae, providing evidence of emergent effects of parental combinations. Results demonstrated parent-specific vertical transmission of specific microbial taxa, highlighting a previously unknown intergenerational effect in this coral species.

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Biology, Microbiology, coral spawning, holobiont, larvae settlement, microbiome, Montipora capitata, restoration

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74 pages

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