Sonoporaton and Impedance Spectroscopy for the Assistance with and Observation of Development of Healthier Embryos and Fetuses.

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dc.contributor.author Matthews, Kainalu M. H.
dc.contributor.department Electrical Engineering
dc.date.accessioned 2019-05-28T19:55:13Z
dc.date.available 2019-05-28T19:55:13Z
dc.date.issued 2017-08
dc.identifier.uri http://hdl.handle.net/10125/62388
dc.subject Sonoporation
dc.subject Impedance spectroscopy
dc.subject Artemia
dc.subject Placenta
dc.subject Gene delivery
dc.subject Ultrasound
dc.title Sonoporaton and Impedance Spectroscopy for the Assistance with and Observation of Development of Healthier Embryos and Fetuses.
dc.type Thesis
dcterms.abstract This thesis presents two separate research projects with an emphasis on developmental biology. Maternal obesity increases the risk for perinatal complications and predisposes for adult disease. Glucose is one of the main nutrients for a fetus, and it has been shown that the placental Glut1 glucose transporter is upregulated (produced in greater quantities) in obese mothers. By employing sonoporation, we can deliver plasmids directly to placental cells to regulate the Glut1 transporter. This work highlights the steps that were taken to optimize the experimental condition such as the ultrasound parameters used for placental sonoporation in mice. Viability testing of embryos for bovine and human in vitro fertilization remains a challenge. The current procedure is to biopsy the blastocyst cells by first puncturing a hole in the embryo, then cultivating two cells from the blastocyst. The cells give information about the possible aneuploidi’s, or an irregular number of chromosomes within a cell, which may exist within the blastocyst. Impedance spectroscopy is a possible alternative to this highly invasive technique. This work looks at impedance spectroscopy employed for the Artemia cysts.
dcterms.description M.S. Thesis. University of Hawaiʻi at Mānoa 2017.
dcterms.language eng
dcterms.publisher University of Hawaiʻi at Mānoa
dcterms.rights All UHM dissertations and theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission from the copyright owner.
dcterms.type Text
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