A Quantitative Cytochemical Analysis on Lysome Fusion in the Digestive Cycle of Paramecium Caudatum

dc.contributor.author Teruya, Julie en_US
dc.contributor.department Biology en_US
dc.date.accessioned 2014-01-15T20:02:19Z
dc.date.available 2014-01-15T20:02:19Z
dc.date.issued 2014-01-15 en_US
dc.description.abstract Cytochemical and ultrastructural studies have been done on the digestive cycle in the protozoa, Paramecium caudatum. Bioassays for digestive vacuole acidification and condensation, and digestive vacuole defecation were established by previous work in this lab. A quantitative cytochemical study on lysosome fusion was lacking. First, a lysosomal-fusion bioassay was devised using fluorescent latex spheres (FPLS) as the digestive vacuole label and acid phosphatase as the lysosome enzyme marker. Using the Gomori technique to localize the acid phosphatase, the amount of enzyme activity was followed against time. The approximate rate of lysosome fusion was then calculated. en_US
dc.format.extent iv, 60 pages en_US
dc.identifier.uri http://hdl.handle.net/10125/31969
dc.publisher University of Hawaii at Manoa en_US
dc.rights All UHM Honors Projects are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission from the copyright owner. en_US
dc.title A Quantitative Cytochemical Analysis on Lysome Fusion in the Digestive Cycle of Paramecium Caudatum en_US
dc.type Term Project en_US
dc.type.dcmi Text en_US
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