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Molecular Systematics and Population Genetics of the Tribe Dacini (Diptera: Tephritidae)

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Title:Molecular Systematics and Population Genetics of the Tribe Dacini (Diptera: Tephritidae)
Authors:San Jose, Michael
Contributors:Rubinoff, Daniel (advisor)
Entomology (department)
Keywords:Entomology
Diptera
geneflow
population genetics
speciation
show 2 moresystematics
Tephritidae
show less
Date Issued:Dec 2018
Publisher:University of Hawaiʻi at Mānoa
Abstract:The tribe Dacini (Diptera; Tephritidae) is a species-rich group with 932 described species. These flies are one of the key tephritid pest groups of Asia and the Pacific causing direct fruit damage, and quarantine restrictions which cause significant economic losses throughout the world. Morphology of this group is constrained due to apparent wasp mimicry limiting the number of variable characters in the groups. This mimicry often confounds evolutionary relationships as cryptic species are abundant within this tribe. As a result, I use molecular methods to understand evolutionary relationships throughout the tribe using both phylogenetic and population genetic approaches.
In Chapter 1, I reconstruct molecular phylogenies of the Tribe Dacini to understand evolutionary relationships between genera and species groups which were based on unique combinations of morphological characters. Phylogenies reconstructed using molecular data confirms the monophyly of Dacus, Bactrocera, and Zeugodacus. However, most groups below the genus level are not monophyletic, and only through further revision will we be able to understand their evolution and clarify the taxonomy within this tribe.
In Chapter 2, I compare the genetic diversity of the mitochondrial gene cytochrome oxidase I in two sister species B. dorsalis and B. carambolae. We analyzed 1,601 individuals from 19 populations using 765 base pairs to infer the haplotype diversity and population structure within and among these two fly species from across their native and invasive ranges. I found no genetic structure between B. dorsalis and B. carambolae and our findings suggest recent and most likely ongoing, genetic exchange between these two species in the wild.
In Chapter 3, I compare genome-wide single nucleotide polymorphisms in two sister species B. dorsalis and B. carambolae. When analyzing these two species, a new cryptic species was discovered in the sympatric range of B. dorsalis and B. carambolae. This diversity was only apparent when analyzing SNPs due to gene flow and potential homoploid hybrid speciation.
Finally, in Chapter 4, I compare genetic diversity found in SNPs across the genome in the native and invasive range of B. dorsalis. Genome-wide SNPs can resolve population structure in the native and invasive range that was not detected in the previous chapter (Chapter 2) using mtDNA sequence data. Bactrocera dorsalis genomics highlights how globalization can influence the genetic diversity and evolutionary pathways of species.
Description:Ph.D. Thesis. University of Hawaiʻi at Mānoa 2018.
Pages/Duration:224 pages
URI:http://hdl.handle.net/10125/62054
Rights:All UHM dissertations and theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission from the copyright owner.
Appears in Collections: Ph.D. - Entomology


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