Genetic Diversity, Development of Improved Diagnostic Assays and Evidence towards the Taro Planthopper (Tarophagus proserpina) as the Vector for Taro Vein Chlorosis Virus (TAVCV) in Hawaii

Gosai, Riten
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[Honolulu] : [University of Hawaii at Manoa], [December 2016]
Taro vein chlorosis virus (TaVCV; genus Nucleorhabdovirus, family Rhabdoviridae) is a recent discovery in Hawaii and causes veinal chlorosis with a netted appearance, stunting and petiole streaking in taro (Colocasia esculenta). Plant death may occur in severe infections. Nucleotide and amino acid sequence comparisons and phylogenetic analyses revealed extremely low levels of genetic diversity in the partial RNA-dependent RNA polymerase (RdRp) gene of 43 Hawaiian and 3 Palauan TaVCV isolates. This sequence information was used to design six new primer pairs targeting different regions of the RdRp gene. Primer set DCGF5/DCGR5 was identified as the most efficient of the six. Following optimization, highly sensitive and robust reverse transcription-polymerase chain reaction (RT-PCR) and immunocapture-RT-PCR (IC-RT-PCR) assays were developed. Localization of TaVCV in insect body parts essential for propagative, circulative virus transmission suggest that the taro planthopper, Tarophagus proserpina, is a vector of TaVCV.
M.S. University of Hawaii at Manoa 2016.
Includes bibliographical references.
Taro vein chlorosis virus, Taro planthopper, Tarophagus proserpina
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