Spatiotemporal Analysis of Distribution Patterns of the Pathogen E. Coli in an Urban Wetland

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2023

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Escherichia coli (E. coli) is a naturally occurring group of bacteria that is ubiquitous throughout aquatic environments and tropical climates. Despite the multitude of natural sources, E. coli may also originate from wastewater, and in high concentrations can lead to severe health issues. This study set out to monitor E. coli concentrations across a wetland that is surrounded by urban land uses, including wastewater infrastructure, cemented stream beds capturing runoff, parking lots, and a highway. The approach to understanding the distribution patterns of E. Coli over space and time was to collect monthly water samples at 20 different locations across the wetland. I hypothesized that E. coli distributions would vary according to proximity to wastewater infrastructure, runoff from parking lots and roads, and springs delivering groundwater to the wetland. On the temporal scale, the hypotheses explored E. coli surges in relation to rainfall events linked to surface runoff, or alternatively, to higher temperatures relating to stagnant water bodies on the wetland. All water samples were processed and analyzed using an IDEXX Colilert 100 mL format system for quantification according to the manufacturer’s instructions. In contrast to predictions, the primary determinant of E. coli concentrations was spatial, with the highest concentrations along the eastern sector, perhaps associated with the indirect connection to an outflow. There was no correlation found with rainfall although there were no major precipitation events during the course of study.

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E. coli, Fecal Bacteria, Urban Wetland

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38

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All UHM dissertations and theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission from the copyright owner.

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Dela Cruz, Brandon

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