Examination of G-banded Human Metaphase Chromosomes by Differential Staining
Date
2014-01-15
Authors
Contributor
Advisor
Department
Instructor
Depositor
Speaker
Researcher
Consultant
Interviewer
Narrator
Transcriber
Annotator
Journal Title
Journal ISSN
Volume Title
Publisher
University of Hawaii at Manoa
Volume
Number/Issue
Starting Page
Ending Page
Alternative Title
Abstract
In this study, isolated human metaphase chromosomes from peripheral blood were subjected to G-banding procedures and stained with a protein specific dye. These chromosome preparations were then viewed with light microscopy and scanning electron microscopy. Chromosomes digested with trypsin and subsequently stained with Coomassie Brilliant Blue R-250, show a banding pattern similar to that of G-bands. SEM micrographs show areas of extensive digestion and areas of preserved morphology corresponding to interbands and bands, respectively. Similar results are obtained by comparing light microscope and SEM micrographs of Giemsa stained chromosomes. These data support the hypothesis that differential organization of nonhistone protein-DNA complexes is responsible for patterns of staining in G-banded chromosomes.
Description
Keywords
Citation
Extent
32 pages
Format
Geographic Location
Time Period
Related To
Related To (URI)
Table of Contents
Rights
All UHM Honors Projects are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission from the copyright owner.
Rights Holder
Local Contexts
Collections
Email libraryada-l@lists.hawaii.edu if you need this content in ADA-compliant format.