Examination of G-banded Human Metaphase Chromosomes by Differential Staining

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2014-01-15
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Griffith, Jody
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University of Hawaii at Manoa
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In this study, isolated human metaphase chromosomes from peripheral blood were subjected to G-banding procedures and stained with a protein specific dye. These chromosome preparations were then viewed with light microscopy and scanning electron microscopy. Chromosomes digested with trypsin and subsequently stained with Coomassie Brilliant Blue R-250, show a banding pattern similar to that of G-bands. SEM micrographs show areas of extensive digestion and areas of preserved morphology corresponding to interbands and bands, respectively. Similar results are obtained by comparing light microscope and SEM micrographs of Giemsa stained chromosomes. These data support the hypothesis that differential organization of nonhistone protein-DNA complexes is responsible for patterns of staining in G-banded chromosomes.
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32 pages
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