Extending Lifespan with Genetic Modification

dc.contributor.advisor Reed, Floyd
dc.contributor.author Holcom, Angelina
dc.contributor.department Biology
dc.date.accessioned 2018-06-28T19:42:36Z
dc.date.available 2018-06-28T19:42:36Z
dc.date.issued 2017-08
dc.description.abstract Aging is a complex biological process that is characterized by different factors. One major factor are telomeres, which cause cell death and results in aging when they shorten. Telomeres are the end caps of the chromosome and are made of a repeated section of DNA. Their purpose is to maintain genomic and cellular stability, which involves protecting the genome from breaking down or mutating. The goal of this experiment is to learn how telomeres function in Drosophila melanogaster in order to better understand their aging process. In the experiment the expression of two genes that produce telomere complex proteins, spindle-E and cav, will be decreased to determine their effect on the life cycle and lifespan. I will measure the life cycle by recording the time it takes for an adult to eclose. The lifespan will be measured by counting how many fruit flies are alive and dead each day. I found that there is an increase in life cycle compared to control and there’s a mutation in eye phenotype of the UAS.Cav.RNAi. x ey-GAL4 population. There is a significant increase in lifespan of the crosses: UAS.Spn-E.RNAi. x Act5C-GAL4, UAS.Spn-E.RNAi. x ey-GAL4, UAS.Cav.RNAi. x Act5C-GAL4, and UAS.Cav.RNAi. x nos-GAL4. The reduction of spindle-E and cav in D.melanogaster appears to affect the ability to develop into adults. There are other telomere genes that can assist in telomere maintenance in the absence of another gene and that these genes have only an effect in specific tissues, which limits their effects in other regions of the organism.
dc.description.degree Biology
dc.format.extent 42 pages
dc.identifier.uri http://hdl.handle.net/10125/56522
dc.publisher University of Hawaii at Manoa
dc.rights All UHM Honors Projects are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission from the copyright owner.
dc.subject aging
dc.subject telomeres
dc.title Extending Lifespan with Genetic Modification
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