The Purification and Molecular Weight of Bovine Enterokinase
| dc.contributor.author | Fong, Lucy | |
| dc.contributor.department | Biology | |
| dc.date.accessioned | 2014-01-15T19:57:19Z | |
| dc.date.available | 2014-01-15T19:57:19Z | |
| dc.date.issued | 2014-01-15 | |
| dc.description.abstract | A purification procedure has been developed for bovine enterokinase. Material extruded from bovine duodena was extracted with 1% Triton-X-100. The extract was purified on a diethyl amino ethyl cellulose column run in the presence of 0.2% Triton. The eluate was loaded on to a pancreatic trypsin inhibitor- Sepharose affinity column and eluted with 0.5M KSCN. The final step of purification is gel filtration on a Sephacryl S-300 column. Yield was about 50% with about 1920 fold purification. | |
| dc.format.extent | iv, 29 pages | |
| dc.identifier.uri | http://hdl.handle.net/10125/31908 | |
| dc.publisher | University of Hawaii at Manoa | |
| dc.rights | All UHM Honors Projects are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission from the copyright owner. | |
| dc.title | The Purification and Molecular Weight of Bovine Enterokinase | |
| dc.type | Term Project | |
| dc.type.dcmi | Text |
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