Epistasis Analysis of Four Genes from Anabaena sp. Strain PCC 7120 Suggests a Connection between PatA and PatS in Heterocyst Pattern Formation

dc.contributor.author Orozco, Christine C.
dc.contributor.author Risser, Douglas D.
dc.contributor.author Callahan, Sean M.
dc.date.accessioned 2016-05-10T03:00:35Z
dc.date.available 2016-05-10T03:00:35Z
dc.date.issued 2005-12
dc.description.abstract The hetR, patA, hetN, and patS genes are part of a regulatory network that regulates the differentiation and patterning of heterocysts in the filamentous cyanobacterium Anabaena sp. strain PCC 7120. In this report, the epistatic interactions of mutant alleles of these four genes have been used to refine our understanding of their relationships to one another. The hetR gene was necessary for differentiation in genetic backgrounds that normally give rise to excessive differentiation, supporting its role as the master regulator of differentiation and indicating that HetR directly regulates factors in addition to hetR and patS genes that regulate differentiation. A functional patS gene was necessary for the delayed multiple-contiguous-heterocyst phenotype observed in hetN mutants as well as for the relative lack of intercalary heterocysts in patA mutants. Epistasis results with mutant alleles of these three genes suggested that PatA attenuates the negative effects of both PatS and HetN on differentiation and promotes differentiation independent of its antagonistic effects on PatS and HetN activity. Cooverxpression of patS and hetR in a synthetic operon indicated that patS acts at a point downstream of hetR transcription in the regulatory network controlling differentiation. A model for the regulation of differentiation that is consistent with these and previous findings is presented. en_US
dc.format.extent 9 en_US
dc.identifier.doi 10.1128/JB.188.5.1808–1816.2006
dc.identifier.uri http://hdl.handle.net/10125/40811
dc.language.iso en-US en_US
dc.publisher American Society for Microbiology en_US
dc.relation.uri http://jb.asm.org/content/188/5/1808.long en_US
dc.rights Copyright © 2006, American Society for Microbiology. All Rights Reserved. en_US
dc.title Epistasis Analysis of Four Genes from Anabaena sp. Strain PCC 7120 Suggests a Connection between PatA and PatS in Heterocyst Pattern Formation en_US
dc.type Article en_US
dc.type.dcmi Text en_US
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