Illustrated Workbook for the Detection of Plant Viruses Infecting Cucurbits: Demonstration of the Use of Direct Immunoblotting Assays

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dc.contributor.author Ullman, Diane E.
dc.contributor.author Cho, John J.
dc.contributor.author Westcot, Daphne M.
dc.contributor.author Custer, Diana M.
dc.date.accessioned 2014-09-17T02:34:17Z
dc.date.available 2014-09-17T02:34:17Z
dc.date.issued 2000-01
dc.description.abstract There are at least 5 viruses of cucurbitaceous plants (squash, melons, cucumber, etc.) that frequently cause severe to complete economic loss for cucurbit producers in the tropics. These viruses are: zucchini yellow mosaic virus (ZYMV), papaya ringspot virus watermelon isolate (PRSV-W, formerly watermelon mosaic virus 1 or WMV I), watermelon mosaic virus 2 (WMV 2), cucumber mosaic virus (CMV) and squash mosaic virus (SqMV). To accurately diagnose virus infections you must use a detection technique. Many techniques are available, including host plant range analysis and serological and molecular analysis. The purpose of our workshop and this illustrated workbook is to demonstrate the use of a serological technique called direct immunoblotting. The advantage of this technique is that it requires very little special equipment, the assay is relatively easy to perform and the assay has some characteristics that make it ideal for diagnosing problems associated with surveying island communities.
dc.description.sponsorship Funded through the U.S. Department of Agriculture Cooperative State Research, Education, and Extension Service Grant 99-38826-7854
dc.format.extent 18 pages
dc.identifier.citation Ullman DE, Cho JJ, Westcot DM, Custer DM. 2000. Illustrated workbook for the detection of plant viruses in cucurbits: demonstration of the use of direct immunoblotting assays. Honolulu (HI): ADAP Project. 18 p. (Agriculture Course Materials; ADAP 92-2)
dc.identifier.uri http://hdl.handle.net/10125/33408
dc.language.iso en-US
dc.publisher Agricultural Development in the American Pacific Project
dc.relation.ispartofseries Agriculture Course Materials
dc.relation.ispartofseries ADAP 92-2
dc.subject plant viruses
dc.subject Cucurbitaceae
dc.subject tropics
dc.subject immunoblotting
dc.subject immunology
dc.subject laboratory techniques
dc.subject educational materials
dc.subject islands
dc.title Illustrated Workbook for the Detection of Plant Viruses Infecting Cucurbits: Demonstration of the Use of Direct Immunoblotting Assays
dc.type Report
dc.type.dcmi Text
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