IASO: Precise genome editing idealizing backbone healing and tale-guided piggyBac

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dc.contributor.advisorOwens, Jesse B.
dc.contributor.authorErrard, Clara
dc.contributor.departmentCell and Molecular Biology
dc.date.accessioned2025-09-30T22:31:57Z
dc.date.issued2025
dc.description.degreeM.S.
dc.embargo.liftdate2026-09-24
dc.identifier.urihttps://hdl.handle.net/10125/111251
dc.subjectGenetics
dc.subjectBiology
dc.subjectnon-homologous end-joining
dc.subjectpiggyBac
dc.subjectTAL
dc.subjecttransposon
dc.titleIASO: Precise genome editing idealizing backbone healing and tale-guided piggyBac
dc.typeThesis
dcterms.abstractGene editing, which is the alteration of the genetic material of a living organism by inserting, replacing or deleting a DNA sequence, could provide a long-term solution for patients suffering from genetic diseases. A major challenge in the field is the efficient and precise insertion of DNA sequences without off-target insertions. While CRISPR/Cas9 has revolutionized genome engineering, its clinical applicability is limited due to potential off-target effects, limited efficiency in dividing cells, and having a limited DNA cargo capacity. Thus, this thesis aims to optimize a novel approach that would result in a specific and efficient method of transferring a gene of interest to a particular target in the genome. We hypothesize that the piggyBac transposon (pB), TALE and non-homologous end joining (NHEJ) can be idealized to target and integrate at a specific DNA target site.
dcterms.extent42 pages
dcterms.languageen
dcterms.publisherUniversity of Hawai'i at Manoa
dcterms.rightsAll UHM dissertations and theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission from the copyright owner.
dcterms.typeText
local.identifier.alturihttps://www.proquest.com/LegacyDocView/DISSNUM/32236622

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