Molecular Characterization of Ti Ringspot Associated Emara Virus and the Development of Assays for Its Detection
Date
2016-12
Authors
Contributor
Advisor
Department
Instructor
Depositor
Speaker
Researcher
Consultant
Interviewer
Narrator
Transcriber
Annotator
Journal Title
Journal ISSN
Volume Title
Publisher
[Honolulu] : [University of Hawaii at Manoa], [December 2016]
Volume
Number/Issue
Starting Page
Ending Page
Alternative Title
Abstract
Ti ringspot disease (TRD) is an emerging virus-like disease of ti plants (Cordyline fruticosa L.) spreading throughout the Hawaiian Islands. Foliar symptoms include chlorotic lesions constricted by secondary veins or circular ring-spots that can coalesce into amorphous lesions. A degenerate reverse transcription (RT)-PCR assay targeting the polymerase gene of known emaraviruses amplified a product from the RNA of symptomatic but not asymptomatic ti plants. The RT-PCR products were sequenced and found to represent a new virus species, designated ti ringspot associated virus (TiRaV). Next generation sequencing of a double-stranded RNA library provided additional sequence information on TiRaV, allowing the development of detection assays able to reliably detect TiRaV and further associate it with TRD. Phylogenetic analyses of this sequence data revealed that TiRaV forms a clade with the emaraviruses Raspberry leaf blotch virus and High Plains wheat mosaic virus in the unassigned plant virus genus Emaravirus.
Description
M.S. University of Hawaii at Manoa 2016.
Includes bibliographical references.
Includes bibliographical references.
Keywords
Citation
Extent
Format
Geographic Location
Time Period
Related To
Theses for the degree of Master of Science (University of Hawaii at Manoa). Plant & Environmental Protection Sciences
Related To (URI)
Table of Contents
Rights
Rights Holder
Local Contexts
Collections
Email libraryada-l@lists.hawaii.edu if you need this content in ADA-compliant format.