Structures of complexes comprised of Fischerella transcription factor HetR with Anabaena DNA targets

dc.contributor.author Kim, Youngchang
dc.contributor.author Ye, Zi
dc.contributor.author Joachimiak, Grazyna
dc.contributor.author Videau, Patrick
dc.contributor.author Young, Jasmine
dc.contributor.author Hurd, Kathryn
dc.contributor.author Callahan, Sean M.
dc.contributor.author Gornicki, Piotr
dc.contributor.author Zhao, Jindong
dc.contributor.author Haselkorn, Robert
dc.contributor.author Joachimiak, Andrzej
dc.date.accessioned 2016-05-04T20:33:23Z
dc.date.available 2016-05-04T20:33:23Z
dc.date.issued 2013-04
dc.description.abstract HetR is an essential regulator of heterocyst development in cyanobacteria. Many mutations in HetR render Anabaena incapable of nitrogen fixation. The protein binds to a DNA palindrome upstream of hetP and other genes. We have determined the crystal structures of HetR complexed with palindromic DNA targets, 21, 23, and 29 bp at 2.50-, 3.00-, and 3.25-Å resolution, respectively. The highest-resolution structure shows fine details of specific protein–DNA interactions. The lower-resolution structures with longer DNA duplexes have similar interaction patterns and show how the flap domains interact with DNA in a sequence nonspecific fashion. Fifteen of 15 protein–DNA contacts predicted on the basis of the structure were confirmed by single amino acid mutations that abolished binding in vitro and complementation in vivo. A striking feature of the structure is the association of glutamate 71 from each subunit of the HetR dimer with three successive cytosines in each arm of the palindromic target, a feature that is conserved among all known heterocyst-forming cyanobacteria sequenced to date. en_US
dc.format.extent 8 en_US
dc.identifier.doi 10.1073/pnas.1305971110
dc.identifier.uri http://hdl.handle.net/10125/40605
dc.language.iso en-US en_US
dc.publisher Proceedings of the National Academy of Sciences of the United States of America en_US
dc.relation.uri http://www.pnas.org/content/110/19/E1716.long en_US
dc.subject heterocyst differentiation en_US
dc.subject Mutagenesis en_US
dc.subject X-ray crystallography en_US
dc.title Structures of complexes comprised of Fischerella transcription factor HetR with Anabaena DNA targets en_US
dc.type Article en_US
dc.type.dcmi Text en_US
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