Safeguarding Anthurium genetic resources: in vitro germplasm bank development and flow cytometry analysis
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2006
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University of Hawaii at Manoa
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This thesis is a compilation, as chapters, of four individual documents under the scope of safeguarding Anthurium genetic resources. The scope of work was to establish in tissue culture a set of Anthurium species of horticultural value, develop the tissue culture protocol necessary for doing so, characterize these and additional species for nuclear DNA content, and then make a subset of species available for distribution via the USDA Ornamental Plant Germplasm Center. The chapters are intended for individual publication as follows: 2) In vitro germplasm bank of Anthurium species: development and techniques. intended for Plant Cell, Tissue and Organ Culture or similar journal in a Research Notes format; 3) Rapid micropropagation of Anthurium species and hybrids by a minimal callus protocol, intended for publication in In Vitro Cellular and Developmental Biology - Plant or similar journal; 4) Anthurium germplasm from Hawai'i: collaboration with the Ornamental Plant Germplasm Center, intended as part of an internet newsletter published by the Ornamental Plant Germplasm Center and targeted towards the lay public. It is included as a chapter to illustrate the variety of communications required for successful in vitro germplasm bank development and as a necessary tool for the increased distribution of germplasm resources; and 5) Nuclear DNA content estimations in Anthurium (Araceae), intended for Plant Systematics and Evolution or similar journal. The format for each chapter is representative of the requirements for their intended publication outlet.
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Anthuriums--Germplasm resources, Gene banks, Plant
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Theses for the degree of Master of Science (University of Hawaii at Manoa). Tropical Plant and Soil Sciences; no. 4084
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