Transport of lysine across the intestine of the freshwater prawn, Macrobrachium rosenbergii
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1975
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Abstract
Two experimental approaches to the analysis of lysine transport by the intestine of the prawn, Macrobrachium rosenbergii, were followed in this study. The first of these yielded information regarding the role in transport of the intestinal mucosal epithelium through the calculated difference between slit intestines, exposed on both sides, and ligated intestines, in which only the serosal side was exposed. The second approach involved perfusion of the intestine mounted as a tube and held in a serosal bath. This study has found that the intestine of the prawn, Macrobrachium rosenbergii, is capable of transporting lysine from the lumen to the blood. This process involves transport of lysine across the apical membrane of the intestinal mucosal epithelial cell by high and low affinity membrane-bound carriers and passage of lysine across the tissue through a paracellular route. The low affinity carrier displays characteristics of an exchange transport system and is not dependent upon extracellular sodium. The high affinity carrier appears to operate at low concentrations of lysine in the intestinal lumen (KT = 18 μM), it is dependent upon anaerobically-derived energy and it has a sodium dependency. Further studies are needed to determine the functional significance and operational characteristics of the paracellular route for lysine movement found in the intestinal epithelium of this prawn. Transmural fluxes of sodium and water were studied as corollaries of lysine movements across the intestinal epithelium. The following relationships were discovered. 1) There is no overall effect of pH on H2O or Na movements. 2) Lysine reduces Jmst of H2O at pH 6.0, but not at 7.5. 3) The reduction in water flux brought about by lysine is significantly intensified by TAP. 4) Lysine does not significantly influence the movement of Na at either pH. 5) TAP reduces water and sodium fluxes only in the presence of lysine. These results suggest that synergistic interactions among pH, lysine and TAP were responsible for alterations in H2O and Na fluxes.
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Typescript.
Bibliography: leaves 117-126.
viii, 126 leaves ill
Bibliography: leaves 117-126.
viii, 126 leaves ill
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Macrobrachium, Lysine in animal nutrition, Lysine
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Theses for the degree of Doctor of Philosophy (University of Hawaii at Manoa). Zoology; no. 803
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