Isolation and characterization of messenger ribonucleic acid for hemoglobin

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dc.contributor.authorLaycock, David Gerald
dc.date.accessioned2009-09-09T19:30:24Z
dc.date.available2009-09-09T19:30:24Z
dc.date.issued1969
dc.descriptionTypescript.
dc.descriptionThesis (Ph. D.)--University of Hawaii, 1969.
dc.descriptionBibliography: leaves 109-114.
dc.descriptionviii, 114 l illus
dc.description.abstractAn 8s ribonucleic acid (RNA) species, isolated from rabbit reticulocytes, has been purified by salt fractionation and analyzed for characteristics of an information-carrying, messenger RNA. This RNA in reticulocytes is restricted to polyribosome complexes which are active in hemoglobin synthesis and has a base composition similar to that expected of a messenger RNA for rabbit globin. This RNA species has been assayed for the ability to support and direct protein synthesis in bacterial extracts. The 8s RNA from reticulocyte polyribosomes is translated when only N-acetyl-valyl- tRNA is present as an initiation residue. Both rabbit globin polypeptide chains have N-terminal valine residues. The protein produced in bacterial extracts directed by this RNA is identical to rabbit globin by chromatography on diethylaminoethyl-cellulose, carboxymethyl-Sephadex C50, paper electrophoresis and gel-filtration on Sephadex G75. The protein product combines with added hemin and the resultant complex is indistinguishable from native rabbit hemoglobin by the above criteria. The profile of tryptic peptides from chromatography on Dowex 50x2 indicates the product is N-acetyl globin. The implications of these results concerning the universality of the genetic code and of protein biosynthetic mechanisms at a macromolecular level are discussed.
dc.identifier.urihttp://hdl.handle.net/10125/11494
dc.language.isoen-US
dc.publisher[Honolulu]
dc.relationTheses for the degree of Doctor of Philosophy (University of Hawaii (Honolulu)). Biomedical Sciences (Genetics - Cell, Molecular and Neuro Sciences); no. 212
dc.rightsAll UHM dissertations and theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission from the copyright owner.
dc.subjectRNA
dc.subjectHemoglobin
dc.titleIsolation and characterization of messenger ribonucleic acid for hemoglobin
dc.typeThesis
dc.type.dcmiText

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