Structure of transcription factor HetR required for heterocyst differentiation in cyanobacteria
Structure of transcription factor HetR required for heterocyst differentiation in cyanobacteria
dc.contributor.author | Kim, Youngchang | |
dc.contributor.author | Joachimiak, Grazyna | |
dc.contributor.author | Ye, Zi | |
dc.contributor.author | Binkowski, T. Andrew | |
dc.contributor.author | Zhang, Rongguang | |
dc.contributor.author | Gornicki, Piotr | |
dc.contributor.author | Callahan, Sean M. | |
dc.contributor.author | Hess, Wolfgang R. | |
dc.contributor.author | Haselkorn, Robert | |
dc.contributor.author | Joachimiak, Andrzej | |
dc.date.accessioned | 2016-05-05T02:06:16Z | |
dc.date.available | 2016-05-05T02:06:16Z | |
dc.date.issued | 2011-04 | |
dc.description.abstract | HetR is an essential regulator of heterocyst development in cyanobacteria. HetR binds to a DNA palindrome upstream of the hetP gene. We report the crystal structure of HetR from Fischerella at 3.0 Å. The protein is a dimer comprised of a central DNA-binding unit containing the N-terminal regions of the two subunits organized with two helix-turn-helix motifs; two globular flaps extending in opposite directions; and a hood over the central core formed from the C-terminal subdomains. The flaps and hood have no structural precedent in the protein database, therefore representing new folds. The structural assignments are supported by sitedirected mutagenesis and DNA-binding studies. We suggest that HetR serves as a scaffold for assembly of transcription components critical for heterocyst development. | |
dc.format.extent | 6 | |
dc.identifier.doi | 10.1073/pnas.1106840108 | |
dc.identifier.uri | http://hdl.handle.net/10125/40610 | |
dc.language.iso | en-US | |
dc.publisher | Proceedings of the National Academy of Sciences of the United States of America | |
dc.relation.uri | http://www.pnas.org/content/108/25/10109.long | |
dc.subject | Anabaena/Fischerella | |
dc.subject | HTH motifs | |
dc.subject | palindrome binding | |
dc.subject | patterned development | |
dc.title | Structure of transcription factor HetR required for heterocyst differentiation in cyanobacteria | |
dc.type | Article | |
dc.type.dcmi | Text |
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