Isolation of a Plasminogen Activating Substance From Commercial Bromelain

Date
2014-01-15
Authors
Cheung, Alan
Contributor
Advisor
Ako, Harry
Department
Biology
Instructor
Depositor
Speaker
Researcher
Consultant
Interviewer
Journal Title
Journal ISSN
Volume Title
Publisher
University of Hawaii at Manoa
Volume
Number/Issue
Starting Page
Ending Page
Alternative Title
Abstract
A procedure has been developed for the isolation of an in vitro plasminogen activating substance from the pineapple plant. A by-product of bromelain production, acetone-still residue, was used as a starting material for an isolation scheme that is initiated with reversed phase chromatography and is followed by ion exchange chromatography. The active substance appears to be a small molecule (less than 5,000 MW) with a polarity similar to aminocyclopentane carboxylic acid, based on its behavior during the above isolation scheme. It is slightly positive at pH 3.8 and is eluted along with "neutral" amino acids. The active substance has no intrinsic proteolytic activity but appears to activate one of the fibrinolytic enzymes. The plasminogen activating substance has also been isolated from commercially available bromelain. Although its mechanism of action in plasminogen activation is not known, this active substance may explain some of the physiological and clinical effects observed in bromelain.
Description
Keywords
Citation
Extent
iv, 32 pages
Format
Geographic Location
Time Period
Related To
Rights
All UHM Honors Projects are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission from the copyright owner.
Rights Holder
Email libraryada-l@lists.hawaii.edu if you need this content in ADA-compliant format.