The Isolation and Characterization of β C-Reactive Protein in neonate Cord Serum, Preparation of Antiserum Against the Isolated β C-Reactive Protein Sample

Siu, Stanton
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University of Hawaii at Manoa
In 1930, Tillet and Francis discovered a serum factor in patients with pneumococcal pneumonia and which reacted with the somatic C-Polysaccharide fraction of the pneumococcal cell wall. This serum factor was called “C-Precipitin” and was later termed “C-Reactive Protein” (CRP), (Abernethy, 1941). C-Reactive Protein was found only in blood from acute phase serum of disease processes involving tissue injury or inflammation. Early studies by Anderson and McCarthy (1950) and Good (1952) utilized the appearance of C-Reactive Protein in serum as a clinical index and means of detection of rheumatic fever. Since then investigators have shown the presence of C-Reactive Protein in ill patients’ serum who have infectious and non-infectious diseases. Successful isolation and purification of C-Reactive Protein was accomplished by Macleod and Avery in 1941, who discovered that his specific abnormal serum protein reacted specifically with the C-Polysaccharide of the pneumoccus in the presence of trace amounts of Ca++ ions. In 1947, McCarty was first to crystallize C-Reactive Protein.
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