An optically addressed thermoresponsive microfluidic system for cell culturing and harvesting

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dc.contributor.authorNamekar, Swapnil Arun
dc.date.accessioned2016-02-19T22:27:14Z
dc.date.available2016-02-19T22:27:14Z
dc.date.issued2012-08
dc.descriptionM.S. University of Hawaii at Manoa 2012.
dc.descriptionIncludes bibliographical references.
dc.description.abstractThe present work demonstrates a "smart" culture platform that can be used for cell culturing and harvesting specific cells, such as stem cells. It is desirable to culture homogeneous specific cell populations for various biomedical applications such as fundamental cellular studies and studies of tissues. Traditional cell culturing techniques lack control over the spatial location of individual cells in a culture, or the types of cells that are seeded. These limitations hinder studies on cell-cell interactions and the creation of completely homogeneous populations of cells. In order to overcome these limitations, a smart culture dish was developed that enables control of specific individual cells in a cell culture by using optical illumination. In addition, a method for seeding specific individual cells in a three-dimensional culture using optical illumination was demonstrated. Both of these techniques can individually be used for achieving control over specific individual cells and their spatial location in a culture. This further enables the culturing and harvesting of specific individual cell types, and the creation of homogeneous specific cell populations.
dc.identifier.urihttp://hdl.handle.net/10125/101000
dc.language.isoeng
dc.publisher[Honolulu] : [University of Hawaii at Manoa], [August 2012]
dc.relationTheses for the degree of Master of Science (University of Hawaii at Manoa). Electrical Engineering.
dc.subjectthermoresponsive microfluidic system
dc.subjectcell culturing
dc.subjectharvesting
dc.titleAn optically addressed thermoresponsive microfluidic system for cell culturing and harvesting
dc.typeThesis
dc.type.dcmiText

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