An optically addressed thermoresponsive microfluidic system for cell culturing and harvesting

Date
2012-08
Authors
Namekar, Swapnil Arun
Contributor
Advisor
Department
Instructor
Depositor
Speaker
Researcher
Consultant
Interviewer
Annotator
Journal Title
Journal ISSN
Volume Title
Publisher
[Honolulu] : [University of Hawaii at Manoa], [August 2012]
Volume
Number/Issue
Starting Page
Ending Page
Alternative Title
Abstract
The present work demonstrates a "smart" culture platform that can be used for cell culturing and harvesting specific cells, such as stem cells. It is desirable to culture homogeneous specific cell populations for various biomedical applications such as fundamental cellular studies and studies of tissues. Traditional cell culturing techniques lack control over the spatial location of individual cells in a culture, or the types of cells that are seeded. These limitations hinder studies on cell-cell interactions and the creation of completely homogeneous populations of cells. In order to overcome these limitations, a smart culture dish was developed that enables control of specific individual cells in a cell culture by using optical illumination. In addition, a method for seeding specific individual cells in a three-dimensional culture using optical illumination was demonstrated. Both of these techniques can individually be used for achieving control over specific individual cells and their spatial location in a culture. This further enables the culturing and harvesting of specific individual cell types, and the creation of homogeneous specific cell populations.
Description
M.S. University of Hawaii at Manoa 2012.
Includes bibliographical references.
Keywords
thermoresponsive microfluidic system, cell culturing, harvesting
Citation
Extent
Format
Geographic Location
Time Period
Related To
Theses for the degree of Master of Science (University of Hawaii at Manoa). Electrical Engineering.
Table of Contents
Rights
Rights Holder
Local Contexts
Email libraryada-l@lists.hawaii.edu if you need this content in ADA-compliant format.