Mechanisms of embryonic and extra-embryonic tissue segregation in pre-implantation embryos

Simple item page
dc.contributor.authorLaeno, Arlene May Apongol
dc.date.accessioned2016-02-19T22:17:51Z
dc.date.available2016-02-19T22:17:51Z
dc.date.issued2012-05
dc.description.abstractThe pre-implantation embryonic stage called blastocyst comprises of inner cell mass (ICM) tissue, which is a mosaic of epiblast (EPI) and primitive endoderm (PE) cells. Prior to uterine implantation, EPI and PE segregate into distinct tissue layers, which will give rise to the fetal body and extra-embryonic structures, respectively. Rhoassociated coiled-coil containing protein kinase (ROCK) is an enzyme responsible for a plethora of cellular functions, including cell migration and cell-to-cell adhesion. To investigate ROCK's role in EPI and PE segregation, blastocysts, prior to tissue segregation, were treated with ROCK inhibitor Y-27632 and immunostained for cell lineage-specific markers. Results showed EPI and PE cells remained intermingled, and ICM cells became loosely associated. Blastocysts treated with Fasudil, another ROCK inhibitor, showed similar results. This suggests that ROCK is required for segregation, possibly by mediating intercellular adhesion to maintain ICM morphology and differential cell adhesion to promote EPI and PE cell sorting.
dc.description.degreeM.S.
dc.identifier.urihttp://hdl.handle.net/10125/101308
dc.languageeng
dc.publisherUniversity of Hawaii at Manoa
dc.relationTheses for the degree of Master of Science (University of Hawaii at Manoa). Developmental and Reproductive Biology.
dc.subjectBlastocyst
dc.titleMechanisms of embryonic and extra-embryonic tissue segregation in pre-implantation embryos
dc.typeThesis
dc.type.dcmiText

Files

Original bundle
Now showing 1 - 2 of 2
No Thumbnail Available
Name:
Laeno_Arlene May_r.pdf
Size:
1.43 MB
Format:
Adobe Portable Document Format
Description:
Version for non-UH users. Copying/Printing is not permitted
Download
No Thumbnail Available
Name:
Laeno_Arlene May_uh.pdf
Size:
1.4 MB
Format:
Adobe Portable Document Format
Description:
Version for UH users
Download

Collections

M.S. - Developmental and Reproductive Biology