Development of an analytical method for β-methylamino-l-alanine, a cyanobacterial metabolite and potential environmental toxin and selective extraction protocol and structure of formamides of β-methylamino-l-alanine (BMAA) from cycas thouarsii
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2014-05
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University of Hawaii at Manoa
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Part I: The development of a qualitative/quantitative analysis of β-Methylamino-L-alanine (BMAA), including a sample preparation protocol and SPE protocol is described. A common interfering metabolite was isolated as the FMOC-derivative. Its structure was determined by spectroscopic methods and confirmed by chemical synthesis. Part II: The qualitative analysis of the BMAA content in samples from cycad leaf and oyster are described. Three different extraction protocols have been developed to categorize the form of BMAA present in either cycad leaf or oyster muscle. The low molecular mass conjugate of β-Methylamino-L-alanine (BMAA) in Cycas thouarsii has been identified as a mixture of regioisomeric formamides. The structures were elucidated on the basis of spectroscopic data and confirmed by chemical synthesis.
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β-Methylamino-L-alanine, Cyanobacteria, Extraction (Chemistry), Organic compounds--Synthesis
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Theses for the degree of Master of Science (University of Hawaii at Manoa). Chemistry.
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