Dose-dependent functions of FGF9 in male primordial germ cell differentiation
Dose-dependent functions of FGF9 in male primordial germ cell differentiation
dc.contributor.author | Ulu, Ferhat | |
dc.date.accessioned | 2016-05-02T22:36:08Z | |
dc.date.available | 2016-05-02T22:36:08Z | |
dc.date.issued | 2013-08 | |
dc.description | M.S. University of Hawaii at Manoa 2013. | |
dc.description | Includes bibliographical references. | |
dc.description.abstract | Primordial Germ Cells (PGCs) are the precursors of gametes. For PGC male differentiation, (i) inhibition of meiosis and (ii) male-inducing factor(s) are essential. It was recently revealed that Fibroblast Growth Factor 9 (FGF9) is one of male-inducing factors. Using isolated PGC culture system, we examined the function of FGF9 in PGC differentiation. We found low FGF9 treatment (0.2 ng/ml) induced PGC male differentiation. Conversely, high FGF9 treatment drastically stimulated PGC proliferation (40%) compared with the control and low FGF9 groups (5~10%). As a result, high FGF9 treatment (25 ng/ml) prevented PGCs to enter the male pathway. Also, we demonstrated that high FGF9 induced ERK1/2 signaling activation for stimulating PGC proliferation while low FGF9 enhanced phosphorylation of p38 signaling to push PGCs into the male pathway. | |
dc.identifier.uri | http://hdl.handle.net/10125/100649 | |
dc.language.iso | eng | |
dc.publisher | [Honolulu] : [University of Hawaii at Manoa], [August 2013] | |
dc.relation | Theses for the degree of Master of Science (University of Hawaii at Manoa). Developmental and Reproductive Biology. | |
dc.subject | Fibroblast Growth Factor 9 | |
dc.title | Dose-dependent functions of FGF9 in male primordial germ cell differentiation | |
dc.type | Thesis | |
dc.type.dcmi | Text |
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