Towards the Development of a Recombinant Expression System for Yeast Reticulon Protein

dc.contributor.author	Kagawa, Allison
dc.date.accessioned	2017-12-18T21:31:43Z
dc.date.available	2017-12-18T21:31:43Z
dc.date.issued	2015-12
dc.description.abstract	Structure determination by X-ray diffraction requires large amounts of purified, functional protein making it especially challenging for membrane proteins due to their low abundance in the cell and instability outside the cell. In this study, we tested the results of our novel screening method for identifying the most promising membrane protein crystallization candidates from native cells through the recombinant expression of the yeast endoplasmic reticulum membrane protein, reticulon(RTN1). Using various mass spectrometry techniques, we were able to determine that RTN1 was not successfully expressed using an E. coli recombinant system, revealing a limitation in the application of our screening method for RTN1.
dc.description.degree	M.S.
dc.identifier.uri	http://hdl.handle.net/10125/51169
dc.language	eng
dc.publisher	University of Hawaii at Manoa
dc.relation	Theses for the degree of Master of Science (University of Hawaii at Manoa). Chemistry
dc.subject	Reticulon
dc.subject	Recombinant
dc.subject	Membrane protein
dc.subject	Mass spectrometry
dc.title	Towards the Development of a Recombinant Expression System for Yeast Reticulon Protein
dc.type	Thesis
dc.type.dcmi	Text

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