Callus and Organized Growth in the Gramineae

Abstract

Linsmaier-Skoog's medium with coconut milk and 2,4-D (2,4-dichlorophenoxy acetic acid) was used to induce callus tissues from the inflorescences of 17 grass species and 3 grass varieties. The Linsmaier-Skoog's medium with only coconut milk was used for the development of callus tissues into organized growth. The technique of using flasks and test tubes was mainly applied for the purpose of callus formation and organized growth from callus. The rotator and auxophyton techniques were also tried in the experiment. An anatomical study was carried out by the paraffin method on callus tissues of one grass species and one grass variety, in which both formed callus tissues, but only the former formed plants from callus tissues.