Isolation of a sugarcane gene promoter and its application to chimeric gene expression in sugarcane protoplasts

Simple item page
dc.contributor.authorBarry, Kelly
dc.date.accessioned2009-07-15T17:23:29Z
dc.date.available2009-07-15T17:23:29Z
dc.date.issued1992
dc.description.abstractThe promoter region of a sugarcane gene has been isolated and characterized from suspension culture cells of Saccharum ssp. hybrid H50-7209. The transcription initiation site was determined by primer extension and physical characteristics such as a 5' transcript splice site, a TATA element, and several upstream repetitive sequences were inferred by sequence analysis. The sugarcane promoter element was isolated by PCR amplification and fused to the GUS reporter gene coding sequence. This chimeric gene construct was transferred to sugarcane protoplasts by electroporation and transient gene expression was monitored by the GUS fluorimetric assay. The sugarcane promoter was approximately five times more effective than the CaMV 35S promoter at driving heterologous gene expression in sugarcane protoplasts.
dc.description.degreePh.D.
dc.identifier.urihttp://hdl.handle.net/10125/9471
dc.languageeng
dc.publisherUniversity of Hawaii at Manoa
dc.relationTheses for the degree of Doctor of Philosophy (University of Hawaii at Manoa). Botanical Sciences (Plant Physiology); no. 2700
dc.rightsAll UHM dissertations and theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission from the copyright owner.
dc.titleIsolation of a sugarcane gene promoter and its application to chimeric gene expression in sugarcane protoplasts
dc.typeThesis
dc.type.dcmiText

Files

Original bundle
Now showing 1 - 2 of 2
No Thumbnail Available
Name:
uhm_phd_9230472_uh.pdf
Size:
3.2 MB
Format:
Adobe Portable Document Format
Description:
Version for UH users
Download
No Thumbnail Available
Name:
uhm_phd_9230472_r.pdf
Size:
3.23 MB
Format:
Adobe Portable Document Format
Description:
Version for non-UH users. Copying/Printing is not permitted
Download

Collections

Ph.D. - Botanical Sciences (Plant Physiology)