Isolation of a sugarcane gene promoter and its application to chimeric gene expression in sugarcane protoplasts
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1992
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University of Hawaii at Manoa
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The promoter region of a sugarcane gene has been isolated and characterized from suspension culture cells of Saccharum ssp. hybrid H50-7209. The transcription initiation site was determined by primer extension and physical characteristics such as a 5' transcript splice site, a TATA element, and several upstream repetitive sequences were inferred by sequence analysis. The sugarcane promoter element was isolated by PCR amplification and fused to the GUS reporter gene coding sequence. This chimeric gene construct was transferred to sugarcane protoplasts by electroporation and transient gene expression was monitored by the GUS fluorimetric assay. The sugarcane promoter was approximately five times more effective than the CaMV 35S promoter at driving heterologous gene expression in sugarcane protoplasts.
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Theses for the degree of Doctor of Philosophy (University of Hawaii at Manoa). Botanical Sciences (Plant Physiology); no. 2700
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