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The Effect of Heavy Metals on the Uptake of L-Histidine by the Polychaete Nereis Succinea

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Item Summary

Title: The Effect of Heavy Metals on the Uptake of L-Histidine by the Polychaete Nereis Succinea
Authors: Peppler, Jessica Elise
Advisor: Whitow, Causey G
Issue Date: Dec 2002
Publisher: University of Hawaii at Manoa
Abstract: Integumentary uptake of 3H-L-Histidine by Nereis succinea was measured in the presence and absence of selected heavy metals and inhibitors in 60% artificial seawater (ASW). At low concentrations of L-Histidine (10 uM), metals stimulated L-Histidine uptake from ASW. Higher concentrations of metal inhibited L-Histidine uptake. In amino acid kinetic experiments, 0.5 uM Zn2+ significantly (P < 0.003) increased both L-Histidine influx Jmax (control: 4.7 ± 0.4; treatment: 15.3 ± 1.7 nmol/g dry weight x 15 min), and Km (control: 23.8 ± 5.1; treatment: 44.0 ± 8.8 uM). Fe3+ (0.5 uM) stimulated influx of 10 uM L-Histidine (Jmax = 6.9 ± 0.4 nmol/g dry wt x 15 min; Km = 86.7 ± 12.3 uM), but neither Ag+ nor Al3+ significantly (P > 0.05) altered amino acid influx. L-Leucine (25 uM) reduced Zn2+ -stimulated L-Histidine influx, suggesting a possible role of the Na-independent L-transport system in metal-stimulated L-Histidine transport by worm integument.
Description: vii, 35 leaves
Rights: All UHM dissertations and theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission from the copyright owner.
Appears in Collections:M.S. - Biomedical Sciences (Physiology)

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