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The Growth Physiology of Pinguiococcus pyrenoidosus With an Emphasis on Fatty Acids

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Item Summary

Title:The Growth Physiology of Pinguiococcus pyrenoidosus With an Emphasis on Fatty Acids
Authors:Messer, Andrea
Contributors:Bidigare, Robert R (advisor)
Oceanography (department)
Date Issued:Dec 2002
Publisher:University of Hawaii at Manoa
Abstract:The Pinguiophyceae are a new class of photosynthetic stramenopiles consisting of five monotypic genera. These chromophyte microalgae have an unusually high percentage of polyunsaturated fatty acids (PUFAs), especially eicosapentaenoic acid (EPA), though the function of these compounds within the cell is unknown. This high PUFA content and composition make these algae favorable candidates for mariculture and for the nutraceutical industry. Chosen for this study was one member of this class, Pinguiococcus pyrenoidosus, which has been observed to possess lipid-like, electron-transparent vacuoles during late stationary phase growth. This study investigated the physiological ecology and fatty acid chemistry of Pinguioco. pyrenoidosus grown in batch and continuous culture. The results revealed that Pinguioco. pyrenoidosus appears to be an obligate phototroph, uses ammonium as a nitrogen source, and can tolerate a wide range of salinity. This alga contains a high concentration of EPA and other PUFAs including docosapentaenoic acid (DPA), which was previously undetected in this species. PUFA content and composition increase at lower temperatures and in late stationary phase growth. These data suggest that Pinguioco. pyrenoidosus utilizes PUFAs as both storage compounds and regulators of membrane fluidity. Maximum PUFA concentration was found in batch culture during late stationary phase growth whereas maximum PUFA production rates were found in continuous culture and reached a plateau at a growth rate of ~50% umax.
Description:x, 77 leaves
Rights:All UHM dissertations and theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission from the copyright owner.
Appears in Collections: M.S. - Oceanography

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