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Characterizing a Novel Connection Between the Plant Hormones Cytokinin and Jasmonic Acid in Control of Maize Leaf Growth.

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Title:Characterizing a Novel Connection Between the Plant Hormones Cytokinin and Jasmonic Acid in Control of Maize Leaf Growth.
Authors:Uyehara, Aimee N.
Contributors:Tropical Plant & Soil Sciences (department)
Keywords:Maize
Jasmonic Acid
Cytokinin
Hsf1
Leaf Growth
Date Issued:Aug 2018
Publisher:University of Hawaiʻi at Mānoa
Abstract:Plant growth is the accumulation of biomass over time and is due the combined effects of cell
division and cell expansion. Understanding the molecular basis of plant growth in the context of the
growthbdefense tradeoff is important for agricultural sciences, and using a simple model to study growth
enables this type of research. In Zea&mays&L. (maize), growth is spatially separated into three distinct
growth zones making the maize leaf base a useful model for investigating growth on multiple scales.
Mutants that affect maize leaf size are useful as an entry into the molecular networks guiding growth. The
semibdominant maize mutant, Hairy&Sheath&Frayed&(Hsf1)&is a CK hypersignaler with reduced leaf size,
fewer dividing cells, and increased levels of the hormone jasmonic acid (JA) in the leaf growth zone. To
determine if increased JA content in&Hsf1&contributes to its reduced leaf growth, the effects of JA on
normal maize leaf growth were characterized. Our results showed that JA treatment dramatically reduced
maize leaf size by reducing growth rate. Reduced JA content in the JA deficient opr7,&opr8 mutant led to
increased leaf size mediated by an increased growth rate. Analysis of epidermal cell counts suggested
that JA reduces cell proliferation rate, since cell size and density were not affected. Exogenous JA
treatments of Hsf1 indicated the mutant has a reduced response to JA. These data help explain the basis
of growth reduction in Hsf1&and set the foundation for further studies to uncover the players that mediate
a novel interaction between CK and JA.
Description:M.S. Thesis. University of Hawaiʻi at Mānoa 2018.
URI:http://hdl.handle.net/10125/62812
Rights:All UHM dissertations and theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission from the copyright owner.
Appears in Collections: M.S. - Tropical Plant and Soil Sciences


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