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Evidence for sap1 as a Virulence Factor in Burkholderia cepacia Complex

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Title:Evidence for sap1 as a Virulence Factor in Burkholderia cepacia Complex
Authors:Bluhm, Andrew
Contributors:Microbiology (department)
Date Issued:Aug 2017
Publisher:University of Hawaiʻi at Mānoa
Abstract:Burkholderia cepacia complex (Bcc) is a consortium of at least 20 closely related Gram
negative species that are a risk factor for cystic fibrosis (CF) patients. Previously in B.
pseudomaelli, a hypothetical protein with no known function, was identified to be a novel
virulence factor and involved in attachment. In this work, highly conserved homologs in Bcc
K56-2 and LO6 were examined in multiple in vitro and in vivo models such as attachment to
eukaryotic cell lines, biofilm attachment and formation, Caenorhabditis elegans survival model,
Drosophila melanogaster feeding model, and mouse lung infection. We found that the deletion
mutants had impaired attachment and biofilm formation, and significantly lower in vivo survival
and replication, compared to the wildtype strains. Finally, C. elegans and mice infected with the
mutants had better survival compared to wildtype infections, supporting the hypothesis that the
protein surface attachment protein 1, or sap1, is a virulence factor.
Description:M.S. Thesis. University of Hawaiʻi at Mānoa 2017.
URI:http://hdl.handle.net/10125/62544
Rights:All UHM dissertations and theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission from the copyright owner.
Appears in Collections: M.S. - Microbiology


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