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DNA Vaccine Expressing Mature Dengue Virus-Like Particles and Serological Tests to Distinguish Dengue and Zika.

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Title:DNA Vaccine Expressing Mature Dengue Virus-Like Particles and Serological Tests to Distinguish Dengue and Zika.
Authors:Youn, Han Ha
Contributors:Biomedical Sciences (Tropical Medicine) (department)
Date Issued:Aug 2017
Publisher:University of Hawaiʻi at Mānoa
Abstract:Our study investigated DNA vaccines expressing mature dengue virus (DENV) viruslike
particles (VLPs) and developed serological tests to distinguish dengue and Zika.
We showed that human-codon optimized DNA vaccines improved the expression
and production of VLPs in vitro compared with non-codon optimized DNA vaccines.
Human-codon optimized DNA vaccine expressing mature VLPs induced similar level
of anti-DENV and neutralizing antibodies compared with that expressing mixed
VLPs. Secondly, we found that DNA-prime and inactivated virion-boost regimens
improved the immunogenicity in mice compared with DNA vaccine alone. Future
challenge experiments are needed to show the protective effect of these vaccine
candidates. Thirdly, we demonstrated that combination of three enzyme-linked
immunosorbent assays based on non-structural protein 1 can distinguish DENV and
Zika virus infections with high specificity and sensitivity. In light of the cross-reactivity
of current serological tests, our findings have implications for serodiagnosis between
DENV and Zika virus or other flaviviral infections.
Description:M.S. Thesis. University of Hawaiʻi at Mānoa 2017.
URI:http://hdl.handle.net/10125/62207
Rights:All UHM dissertations and theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission from the copyright owner.
Appears in Collections: M.S. - Biomedical Sciences (Tropical Medicine)


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